Journal of Physiology-Paris

Shimahara T
Journal of Physiology-Paris - vol. 99 73-74 (2006)

Probing cell-division phenotype space and Polo-like kinase function using small molecules.

Nature chemical biology

Peters U, Cherian J, Kim JH, Kwok BH, Kapoor TM
Nature chemical biology - vol. 2 618-626 (2006)

Cell-permeable small molecules that inhibit their targets on fast timescales are powerful probes of cell-division mechanisms. Such inhibitors have been identified using phenotype-based screens with chemical libraries. However, the characteristics of compound libraries needed to effectively span cell-division phenotype space, to find probes that target different mechanisms, are not known. Here we show that a […]

Integration of virtual and physical screening

Drug Discovery Today: Technologies

Fara DC, Oprea TI, Prossnitz ER, Bologa CG, Edwards BS, Sklar LA
Drug Discovery Today: Technologies - vol. 3 377-385 (2006)

High-throughput screening (HTS) represents the dominant technique for the identification of new lead compounds in current drug discovery. It consists of physical screening (PS) of large libraries of chemicals against one or more specific biological targets. Virtual screening (VS) is a strategy for in silico evaluation of chemical libraries for a given target, and can […]

Identification of novel pharmacological activities of an antifungal agent, nystatin, to promote dendritic cell maturation

J Invest Dermatol

Ogawa Y, Mizumoto N, Tanaka H, Matsushima H, Takashima A
J Invest Dermatol - vol. 126 349-353 (2006)

As an unbiased functional screen to identify agents activating dendritic cells (DCs), we recently developed a DC-based biosensor system, in which a stable murine DC line XS106 was engineered to express the yellow fluorescent protein (YFP) gene under the control of the IL-1beta promoter. Here we report that nystatin (NYT), an antifungal drug of the […]

A High-Throughput Drug Screen Targeted to the 5’Untranslated Region of Alzheimer Amyloid Precursor Protein mRNA

Journal of Biomolecular Screening

Bandyopadhyay S
Journal of Biomolecular Screening - vol. 11 469-480 (2006)

Managing, profiling and analyzing a library of 2.6 million compounds gathered from 32 chemical providers

Molecular Diversity

Monge A, Arrault A, Marot C, Morin-Allory L
Molecular Diversity - vol. 10 389-403 (2006)

The data for 3.8 million compounds from structural databases of 32 providers were gathered and stored in a single chemical database. Duplicates are removed using the IUPAC International Chemical Identifier. After this, 2.6 million compounds remain. Each database and the final one were studied in term of uniqueness, diversity, frameworks, ‘drug-like’ and ‘lead-like’ properties. This […]

Discovery of novel immunostimulants by dendritic-cell – based functional screening


Mizumoto N, Gao J, Matsushima H, Ogawa Y, Tanaka H, Takashima A
Blood - vol. 106 3082-3090 (2005)

A High-Throughput Screen to Identify Inhibitors of Amyloid beta-Protein Precursor Processing

Journal of Biomolecular Screening

Bakshi P, Liao Y, Gao J, Ni J, Stein R, Yeh L, Wolfe MS
Journal of Biomolecular Screening - vol. 10 1-12 (2005)

Integration of Virtual Screening with High-Throughput Flow Cytometry to Identify Novel Small Molecule Formylpeptide

Molecular pharmacology

Edwards BS, Bologa C, Young SM, Balakin KV, Prossnitz ER, Savchuck NP, Sklar LA, Oprea TI
Molecular pharmacology - vol. 68 1301-1310 (2005)

Receptor Binding Techniques

Nethods in Molecular Biology

Tobergte DR, Curtis S
Nethods in Molecular Biology - vol. Second Edi 17-25 (2005)

applicability for this approach.