Publications


Publications
Flow cytometry for high-throughput, high-content screening

Current Opinion in Chemical Biology

Edwards BS, Oprea T, Prossnitz ER, Sklar LA
Current Opinion in Chemical Biology - vol. 8 392-398 (2004)

Flow cytometry is a mature platform for quantitative multi-parameter measurement of cell fluorescence. Recent innovations allow up to 30-fold faster serial processing of bulk cell samples. Homogeneous discrimination of free and cell-bound fluorescent probe eliminates wash steps to streamline sample processing. Compound screening throughput may be further enhanced by multiplexing of assays on color-coded bead […]

Publications
Prednisone reduces muscle degeneration in dystrophin-deficient Caenorhabditis elegans

Neuromuscular Disorders

Gaud A, Simon JM, Witzel T, Carre-Pierrat M, Wermuth CG, Ségalat L
Neuromuscular Disorders - vol. 14 365-370 (2004)

Duchenne muscular dystrophy is a degenerative muscular disease caused by mutations in the dystrophin gene. There is no curative treatment against Duchenne muscular dystrophy. In several countries, the steroid prednisone (or analogs) is prescribed as a palliative treatment. In the model animal Caenorhabditis elegans, mutations of the dys-1 dystrophin-like gene lead to a muscular degenerative […]

Publications
Development of an Assay to Screen for Inhibitors of Tau Phosphorylation by Cdk5

Journal of Biomolecular Screening

Ahn JS
Journal of Biomolecular Screening - vol. 9 122-131 (2004)

Publications
High-throughput target validation in model organisms

Drug Discovery Today: TARGETS

Doan T
Drug Discovery Today: TARGETS - vol. 3 191-197 (2004)

Modern drug discovery includes a progression from the identification of molecular targets pertinent to disease processes to the validation of those targets and compound screening to modulate the targets of interest.To save time and reduce cost,analysis of gene function can be rapidly assessed in model organisms using several approaches, including mutagenesis, antisense knockdown and chemical […]

Publications
Functional Electrical Stimulation and rehabilitation—an hypothesis

Medical Engineering & Physics

Rushton D
Medical Engineering & Physics - vol. 25 75-78 (2003)

Functional Electrical Stimulation (FES), used to mimic a weak or paralysed movement, sometimes is followed by a specific recovery of voluntary power in that movement. The mechanism by which this occurs is unclear, and the presumption has often been that FES may somehow promote adaptive changes in cortical connectivity. However, the unique feature of electrical […]

Publications
Virtual screening in lead discovery: A viewpoint

Molecules

Oprea TI
Molecules - vol. 7 51-62 (2002)

Virtual screening (VS) methods have emerged as an adaptive response to massive throughput synthesis and screening technologies. Based on the structure-permeability paradigm, the Lipinski rule of five has become a standard property filtering protocol for VS. Three possible VS scenarios with respect to optimising binding affinity and pharmacokinetic properties are discussed. The parsimony principle for […]

Publications
How to acquire new biological activities in old compounds by computer prediction

Journal of Computer-Aided Molecular Design

Poroikov VV, Filimonov DA
Journal of Computer-Aided Molecular Design - vol. 16 819-824 (2002)

Due to the directed way of testing chemical compounds’ in drug research and development many projects fail because serious adverse effects and toxicity are discovered too late, and many existing prospective activities remain unstudied. Evaluation of the general biological potential of molecules is possible using a computer program PASS that predicts more than 780 pharmacological […]

Publications
Virtual screening in lead discovery: A viewpoint

Molecules

Oprea TI
Molecules - vol. 7 51-62 (2002)

Virtual screening (VS) methods have emerged as an adaptive response to massive throughput synthesis and screening technologies. Based on the structure-permeability paradigm, the Lipinski rule of five has become a standard property filtering protocol for VS. Three possible VS scenarios with respect to optimising binding affinity and pharmacokinetic properties are discussed. The parsimony principle for […]