Methylxanthine drugs are chitinase inhibitors: Investigation of inhibition and binding modes

Chemistry and Biology

Rao FV, Andersen OA, Vora KA, DeMartino JA, Van Aalten DMF
Chemistry and Biology - vol. 12 973-980 (2005)

Family 18 chitinases play key roles in a range of pathogenic organisms and are overexpressed in the asthmatic lung. By screening a library of marketed drug molecules, we have identified methylxanthine derivatives as possible inhibitor leads. These derivatives, theophylline, caffeine, and pentoxifylline, are used therapeutically as antiinflammatory agents, with pleiotropic mechanisms of action. Here it […]

HERG-Lite??: A novel comprehensive high-throughput screen for drug-induced hERG risk

Journal of Pharmacological and Toxicological Methods

Wible BA, Hawryluk P, Ficker E, Kuryshev YA, Kirsch G, Brown AM
Journal of Pharmacological and Toxicological Methods - vol. 52 136-145 (2005)

Introduction: Direct block of IKr by non-antiarrhythmic drugs (NARDs) is a major cause of QT prolongation and torsades de pointes (TdP), and has made the hERG potassium channel a major target of drug safety programs in cardiotoxicity. Block of hERG currents is not the only way that drugs can adversely impact the repolarizing current IKr, […]

Development of a mechanism-based assay for tissue transglutaminase – Results of a high-throughput screen and discovery of inhibitors

Analytical Biochemistry

Case A, Ni J, Yeh LA, Stein RL
Analytical Biochemistry - vol. 338 237-244 (2005)

Tissue transglutaminase (TGase) is a Ca2+-dependent enzyme that catalyzes cross-linking of intracellular proteins through a mechanism that involves isopeptide bond formation between Gln and Lys residues. In addition to its transamidation activity, TGase can bind guanosine 5???-triphosphate (GTP) and does so in a manner that is antagonized by calcium. Once bound, GTP undergoes hydrolysis to […]

Multi-parameter high throughput screening assays (MPHTS)


Altar CA, Brockman JA, Evans D, Hook DJ, Klimczak LJ, Laeng P, Palfreyman M, Rajan P
Society - vol. 9 51 (2005)

The present invention relates to screening methods and assays that are referred to herein as multi-parameter hight throughput screening (MPHTS) assays. These MPHTS assays are useful for identifying candidate pharmaceutical compounds. In particular, the screening methods of this invention may be used to identify compounds that have potential therapeutic bene?ts for the treatment of neurop […]

High-Throughput Screening with HyperCyt(R) Flow Cytometry to Detect Small Molecule Formylpeptide Receptor Ligands

Journal of Biomolecular Screening

Young SM
Journal of Biomolecular Screening - vol. 10 374-382 (2005)

Discovery of novel immunostimulants by dendritic-cell – based functional screening


Mizumoto N, Gao J, Matsushima H, Ogawa Y, Tanaka H, Takashima A
Blood - vol. 106 3082-3090 (2005)

A High-Throughput Screen to Identify Inhibitors of Amyloid beta-Protein Precursor Processing

Journal of Biomolecular Screening

Bakshi P, Liao Y, Gao J, Ni J, Stein R, Yeh L, Wolfe MS
Journal of Biomolecular Screening - vol. 10 1-12 (2005)

Integration of Virtual Screening with High-Throughput Flow Cytometry to Identify Novel Small Molecule Formylpeptide

Molecular pharmacology

Edwards BS, Bologa C, Young SM, Balakin KV, Prossnitz ER, Savchuck NP, Sklar LA, Oprea TI
Molecular pharmacology - vol. 68 1301-1310 (2005)

Development of an Assay to Screen for Inhibitors of Tau Phosphorylation by Cdk5

Journal of Biomolecular Screening

Ahn JS
Journal of Biomolecular Screening - vol. 9 122-131 (2004)

High-throughput target validation in model organisms

Drug Discovery Today: TARGETS

Doan T
Drug Discovery Today: TARGETS - vol. 3 191-197 (2004)

Modern drug discovery includes a progression from the identification of molecular targets pertinent to disease processes to the validation of those targets and compound screening to modulate the targets of interest.To save time and reduce cost,analysis of gene function can be rapidly assessed in model organisms using several approaches, including mutagenesis, antisense knockdown and chemical […]