A high-throughput screen for aggregation-based inhibition in a large compound library

Journal of Medicinal Chemistry

Feng BY, Simeonov A, Jadhav A, Babaoglu K, Inglese J, Shoichet BK, Austin CP
Journal of Medicinal Chemistry - vol. 50 2385-2390 (2007)

High-throughput screening (HTS) is the primary technique for new lead identification in drug discovery and chemical biology. Unfortunately, it is susceptible to false-positive hits. One common mechanism for such false-positives is the congregation of organic molecules into colloidal aggregates, which nonspecifically inhibit enzymes. To both evaluate the feasibility of large-scale identification of aggregate-based inhibition and […]

Understanding false positives in reporter gene assays: In silico chemogenomics approaches to prioritize cell-based HTS data

Journal of Chemical Information and Modeling

Crisman TJ, Parker CN, Jenkins JL, Scheiber J, Thoma M, Kang ZB, Kim R, Bender A, Nettles JH, Davies JW, Glick M
Journal of Chemical Information and Modeling - vol. 47 1319-1327 (2007)

High throughput screening (HTS) data is often noisy, containing both false positives and negatives. Thus, careful triaging and prioritization of the primary hit list can save time and money by identifying potential false positives before incurring the expense of followup. Of particular concern are cell-based reporter gene assays (RGAs) where the number of hits may […]

A Cell-Based Assay for I κ B α Stabilization Using A Two-Color Dual Luciferase-Based Sensor

ASSAY and Drug Development Technologies

Davis RE, Zhang Y, Southall N, Staudt LM, Austin CP, Inglese J, Auld DS
ASSAY and Drug Development Technologies - vol. 5 85-104 (2007)

Antifungal chemical compounds identified using a C. elegans pathogenicity assay

PLoS Pathogens

Breger J, Fuchs BB, Aperis G, Moy TI, Ausubel FM, Mylonakis E
PLoS Pathogens - vol. 3 0168-0178 (2007)

There is an urgent need for the development of new antifungal agents. A facile in vivo model that evaluates libraries of chemical compounds could solve some of the main obstacles in current antifungal discovery. We show that Candida albicans, as well as other Candida species, are ingested by Caenorhabditis elegans and establish a persistent lethal […]

A Single Mutation in Chikungunya Virus Affects Vector Specificity and Epidemic Potential

Tsetsarkin KA, Vanlandingham DL, Mcgee CE, Higgs S
- vol. 3 (2007)

Infection with chikungunya virus in Italy: an outbreak in a temperate region

The Lancet

Rezza G, Nicoletti L, Angelini R, Romi R, Finarelli A, Panning M, Cordioli P, Fortuna C, Boros S, Magurano F, Silvi G, Angelini P, Dottori M, Ciufolini M, Majori G, Cassone A
The Lancet - vol. 370 1840-1846 (2007)

BACKGROUND Chikungunya virus (CHIKV), which is transmitted by Aedes spp mosquitoes, has recently caused several outbreaks on islands in the Indian Ocean and on the Indian subcontinent. We report on an outbreak in Italy. METHODS After reports of a large number of cases of febrile illness of unknown origin in two contiguous villages in northeastern […]

A Novel Inhibitor of Mycobacterium tuberculosis

Journal of Biomolecular Screening

White EL, Southworth K, Ross L, Cooley S, Gill RB, Sosa MI, Rasmussen L, Goulding C, Eisenberg D, Fletcher TM
Journal of Biomolecular Screening 100-105 (2007)

Drug Repurposing

MSI Consultancy

Stuart-Kregor P
MSI Consultancy (2007)

Development of a Fission Yeast-Based High-Throughput Screen to Identify Chemical Regulators of cAMP Phosphodiesterases

Journal of Biomolecular Screening

Ivey FD, Wang L, Demirbas D, Allain C, Hoffman CS
Journal of Biomolecular Screening - vol. 13 62-71 (2007)

Identification of Tau Stem Loop RNA Stabilizers

Journal of Biomolecular Screening

Donahue CP, Ni J, Rozners E, Glicksman MA, Wolfe MS
Journal of Biomolecular Screening - vol. 12 789-799 (2007)

Alternative splicing of tau exon 10 produces tau isoforms with either 3 (3R) or 4 (4R) repeated microtubule-binding domains. Increased ratios of 4R to 3R tau expression, above the physiological 1:1, leads to neurofibrillary tangles and causes neurode-generative disease. An RNA stem loop structure plays a significant role in determining the ratio, with decreasing stability […]