The 2019 Garrod Lecture: MDR efflux in Gram-negative bacteria – How understanding resistance led to a new tool for drug discovery

Journal of Antimicrobial Chemotherapy

Piddock LJ
Journal of Antimicrobial Chemotherapy - vol. 74 3128-3134 (2019)

The AcrAB-TolC MDR efflux system confers intrinsic MDR and overproduction confers clinically relevant resistance to some antibiotics active against Gram-negative bacteria. The system is made up of three components, namely AcrA, AcrB and TolC, otherwise known as the AcrAB-TolC tripartite system. Inactivation or deletion of a gene encoding one of the constituent proteins, or substitution […]

Repurposing of ribavirin as an adjunct therapy against invasive Candida strains in an in vitro study

Antimicrobial Agents and Chemotherapy

Yousfi H, Cassagne C, Ranque S, Rolain JM, Bittar F
Antimicrobial Agents and Chemotherapy - vol. 63 1-8 (2019)

The use of antifungal agents in clinical settings is limited by the appearance of drug resistance and adverse side effects. There is, therefore, an urgent need to develop new drugs to strengthen the treatment of invasive fungal diseases. The aim of this study is to describe the potential repurposing of ribavirin as an adjunct therapy […]

A Small Molecule Targeting Mutagenic Translesion


Wojtaszek JL, Chatterjee N, Najeeb J, Hong J, Walker GC, Zhou P
Cell - vol. 178 152-159.e11 (2019)

Novel drug candidates for treating esophageal carcinoma: A study on differentially expressed genes, using connectivity mapping and molecular docking

International Journal of Oncology

Chen YT, Xie JY, Sun Q, Mo WJ
International Journal of Oncology - vol. 54 152-166 (2019)

Patients with esophageal carcinoma (ESCA) have a poor prognosis and high mortality rate. Although standard therapies have had effect, there is an urgent requirement to develop novel options, as increasing drug tolerance has been identifiedinclinicalpractice.Inthepresentstudy,differentially expressedgenes(DEGs)ofESCAwereidentifiedinTheCancer Genome Atlas and Genotype-Tissue Expression databases. Functional and protein-protein interaction (PPI) analyses were performed. The Connectivity Map (CMAP) was […]

Utilisation of the Prestwick Chemical Library to identify drugs that inhibit the growth of mycobacteria


Kanvatirth P JR
PLoS ONE - vol. 14 1-40 (2019)

Utilisation of the Prestwick Chemical Library to identify drugs that inhibit the growth of mycobacteria


Kanvatirth P, Jeeves RE, Bacon J, Besra GS, Alderwick LJ
PLoS ONE - vol. 14 (2019)

Tuberculosis (TB) is an infectious bacterial disease that kills approximately 1.3 million people every year. Despite global efforts to reduce both the incidence and mortality associated with TB, the emergence of drug resistant strains has slowed any progress made towards combating the spread of this deadly disease. The current TB drug regimen is inadequate, takes […]

Identification and characterization of menadione and benzethonium chloride as potential treatments of Pierce’s disease of grapevines


Zhang S, Jain M, Fleites LA, Rayside PA, Gabriel DW
Phytopathology - vol. 109 233-239 (2019)

Xylella fastidiosa infects a wide range of plant hosts and causes Pierce’s disease (PD) of grapevines. The type 1 multidrug resistance (MDR) efflux system is essential for pathogenicity and survival of bacterial pathogens in planta. X. fastidiosa, with a single MDR system, is significantly more vulnerable to inhibition by small-molecule treatments than most bacterial pathogens […]

Screening and evaluation of antiviral compounds against Equid alpha-herpesviruses using an impedance-based cellular assay


Thieulent CJ, Hue ES, Fortier CI, Dallemagne P, Zientara S, Munier-Lehmann H, Hans A, Fortier GD, Pitel PH, Vidalain PO, Pronost SL
Virology - vol. 526 105-116 (2019)

Equid alpha-herpesviruses (EHV) are responsible for different diseases in equine population. EHV-1 causes respiratory diseases, abortions and nervous disorders, EHV-4 causes respiratory diseases and sporadic abortion, while EHV-3 is responsible of equine coital exanthema. In view of the lack of efficacy of vaccines against EHV-1 and EHV-4 and in the absence of vaccines against EHV-3, […]

Drug‐induced increase in lysobisphosphatidic acid reduces the cholesterol overload in Niemann–Pick type C cells and mice

EMBO reports

Moreau D, Vacca F, Vossio S, Scott C, Colaco A, Paz Montoya J, Ferguson C, Damme M, Moniatte M, Parton RG, Platt FM, Gruenberg J
EMBO reports - vol. 20 1-15 (2019)

Most cells acquire cholesterol by endocytosis of circulating low-density lipoproteins (LDLs). After cholesteryl ester de-esterification in endosomes, free cholesterol is redistributed to intracellular membranes via unclear mechanisms. Our previous work suggested that the unconventional phospholipid lysobisphosphatidic acid (LBPA) may play a role in modulating the cholesterol flux through endosomes. In this study, we used the […]

Fibrates as drugs with senolytic and autophagic activity for osteoarthritis therapy


Nogueira-Recalde U, Lorenzo-Gómez I, Blanco FJ, Loza MI, Grassi D, Shirinsky V, Shirinsky I, Lotz M, Robbins PD, Domínguez E, Caramés B
EBioMedicine - vol. 45 588-605 (2019)

Background: Ageing-related failure of homeostasis mechanisms contributes to articular cartilage degeneration and osteoarthritis (OA), for which disease-modifying treatments are not available. Our objective was to identify molecules to prevent OA by regulating chondrocyte senescence and autophagy. Methods: Human chondrocytes with IL-6 induced senescence and autophagy suppression and SA-β-gal as a reporter of senescence and LC3 […]