Development of a Cost-Effective and Low-Toxicity Safranin-Based Assay for Discovering Candida Albicans Biofilm Formation Inhibitors in Large-Scale Screening Campaigns

Il y a 3 jours

Screening assays for discovering Candida albicans biofilm inhibitors typically measure either metabolic activity or attached biomass. Although biomass staining with Crystal Violet or Safranin is affordable and easy to perform, standard protocols often require toxic solvents for biofilm fixation and dye extraction. We developed and evaluated an alternative Safranin staining method that uses heat fixation […]

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